Optimization of Glucose Recovery from Saccharomyces cerevisiae Waste via Hydrolysis Using RSM
DOI:
https://doi.org/10.70609/gtech.v9i2.6581Keywords:
Optimization, Hydrolysis, Glucose, RSM, WasteAbstract
The liquid waste resulting from the regeneration of Saccharomyces cerevisiae contains a significant amount of starch, which has the potential to be optimized for glucose production. This study aims to optimize glucose levels from the liquid waste using hydrolysis with the Response Surface Methodology (RSM). The tested variables include the volume of Saccharomyces cerevisiae regeneration liquid waste (mL): 300; 600; 900; 1200; and 1500, as well as water volume (mL): 200; 400; 600; 800; and 1000. RSM with a Central Composite Design (CCD) requires 13 experimental data combinations of variables. The hydrolysis results were analyzed using RSM to determine the effect of variables and their interactions on glucose content. Optimization of glucose levels using RSM resulted in an optimal glucose content of 23.5% at a liquid waste volume of 1378.79 mL and a water volume of 1.5238 mL, based on an analysis using Minitab 19 software. Glucose content testing from 25 data points showed the highest level at 27% for a liquid waste volume of 900 mL and a water volume of 600 mL, while spectrophotometry recorded a peak glucose content of 29.7% under the same variables. The findings of this study can be the basis for developing a more efficient hydrolysis process that produces glucose which can help industry to make waste processing methods more efficient and increase the added value of by-products.
References
Azizah, Y. and Marziah, A., 2022. Hidrolisis Ampas Tebu (Baggase) Menggunakan HCl Menjadi Cellulosa Powder. Jurnal Inovasi Ramah Lingkungan, 3(3), pp.11-15.
Bachtiar, S., Wahyuningtiyas, R. and Sari, N.K., 2021. Bioetanol Dari Limbah Cair Tepung Terigu Dengan Proses fermentasi Menggunakan Turbo Yeast. Jurnal Teknik Kimia, 16(1), pp.29-34.
Chavan, R., Saxena, K., & Tigote, D. 2015. Optimization of acid hydrolysis process for free glucose recovery from starch. International Journal of Innovative Science, Engineering & Technology, 2(12), 55-58.
Ellouze, I., Akhavan, N., Singar, S., Dawkins, K., Nagpal, R., & Arjmandi, B., 2023. The Relationship of Fruits and Fruit-Products Consumption with Glucose Homeostasis and Diabetes: A Comprehensive Update on the Current Clinical Literature. Dietetics, 2(3), 237-266.
Enero 2023, Profil Perusahaan PT. Energi Agro Nusantara (ENERO), (https://enero.co.id/tentang-kami-2/), diakses pada tanggan 07 Maret 2023.
Djimtoingar, S. S., Derkyi, N. S. A., Kuranchie, F. A., & Yankyera, J. K. (2022). A review of response surface methodology for biogas process optimization. Cogent Engineering, 9(1), 2115283.
Faridah, D.N. and Thonthowi, A., 2020. Karakterisasi Fisik Pati Tapioka Modifikasi Gabungan Hidroksipropilasi dengan Fosfat-Ikat Silang. Jurnal Mutu Pangan: Indonesian Journal of Food Quality, 7(1), pp.30-37.
Farrag, N. M., Gadalla, M. A., & Fouad, M. K. (2022). Base-Catalyzed Transesterification vs. Supercritical Methanol Transesterification in the Optimization, Rigorous Model Simulation, and Heat Integration for Biodiesel Production. Journal of Hunan University Natural Sciences, 49(4), 193–205.
Gargazi, G., Hendrawani, H., & Hulyadi, H. (2022). Identifikasi Karakter Biodisel Minyak Jelantah Menggunakan Instrumen Gas Cromatografi Mass Spectroscopy (GC-MS). Empiricism Journal, 3(2), 333–340.
Golachowski, A., Drożdż, W., Golachowska, M., Kapelko-Żeberska, M., & Raszewski, B. 2020). Production and properties of starch citrates—Current research. Foods, 9(9), 1311
Hadiyat, M. A., Sopha, B. M., & Wibowo, B. S., 2022. Response surface methodology using observational data: a systematic literature review. Applied Sciences, 12(20), 10663.
Iryani, A.S., 2020. Pengaruh Jenis Katalis Asam Terhadap Studi Kinetika Proses Hidrolisis Pati Dalam Ubi Kayu, Ilmu Teknik, 8(15), pp.1078-1081
Mardina, P., Prathama, H. A., & Hayati, D. M. 2014. Pengaruh waktu hidrolisis dan konsentrasi katalisator asam sulfat terhadap sintesis furfural dari jerami padi. Konversi, 3(2), 1-8.
Montgomery, D. C. (2017). Design and analysis of experiments. John wiley & sons.
Muzakhar, S. S. A., Assidiqie, G. I., Siregar, A. S. B., Aparamarta, H. W., Fahmi., & Gunawan, S., 2023. Optimization of Esterification in the Synthesis of Surfactants Feedstock from Polar Lipid Fraction of Crude Palm Oil. Journal of Fundamentals and Applications of Chemical Engineering (JFAChE), 4(2), 30–39.
Nabilah .A., Chasanah, Y.H.N. and Sari, N.K., 2023. Optimasi Kadar Bioetanol Dari Limbah Cair Tepung Terigu Menggunakan Response Surface Method (RSM). Jurnal Inovasi Teknik Kimia, 8(3), pp.155-159.
Praputri, E., Sundari, E., Firdaus, F., & Sofyan, S. (2018). Penggunaan katalis homogen dan heterogen pada proses hidrolisis pati umbi singkong karet menjadi glukosa. Indonesian Journal of Industrial Research, 8(2), 105-110.
Ramaraj, R, Unpaprom, Y, 2019, ‘Optimization of Pretreatment Condition for Ethanol Production from Cyperus Diformis by Response Surface Methodology’, Journal of Science and Technology, 9(218), pp.5-9.
Sari, N. K., Ernawati, D., & Sari, K. N., 2024. Optimization of Glucose from Saccaromycess cerevicae Liquid Waste Using the Acid Hydrolysis Process. Nusantara Science and Technology Proceedings, 13-17.
Satoh, T., 2014, Molecular mechanisms for the regulation of insulin-stimulated glucose
Suryati, S., Meriatna, M., Masrullita, M., Safriwardy, F., & Ardiansyah, A., 2021, Optimum Temperature and Time for Acid Hydrolysis in Reducing Sugar Manufacturing from Arabica Coffee Cascara with Response Surface Methodology (RSM). International Journal for Educational and Vocational Studies, 3(2), 163-168.
Susmiati, Y., Setyaningsih, D., & Sunarti, T. C. 2011. Rekayasa proses hidrolisis pati dan serat ubi kayu (Manihot utilissima) untuk produksi bioetanol. Agritech, 31(4).
Świątek, K., Gaag, S., Klier, A., Kruse, A., Sauer, J., & Steinbach, D. 2020. Acid hydrolysis of lignocellulosic biomass: Sugars and furfurals formation. Catalysts, 10(4), 437.
Yadav, P., & Majumder, C. B. 2017. Production of glucose syrup by the hydrolysis of starch made from rotten potato. Journal of Integrated Science and Technology, 5(1), 19-22.
Yu, H., Fang, Q., Cao, Y., & Liu, Z., 2016, Effect of HCl on starch structure and properties of starch-based wood adhesives. BioResources, 11(1), 1721-1728.
Zani, dkk, 2019, ‘Optimization of Process Parameters for Bioetanol Production from Oil Palm Frond Juice by Saccharomyces cerevisiae Using Response Surface Methodology As A Tool’, IOP, hh. 10
Zulnazri, Z., Dewi, R., Muarif, A., Fikri, A., Fithra, H., Roesyadi, A.,& Alva, S., 2024. Effect of Hydrochloric Acid Hydrolysis under Sonication and Hydrothermal Process to Produce Cellulose Nanocrystals from Oil Palm Empty Fruit Bunch (OPEFB). Polymers, 16(13), 1866.
Downloads
Published
Issue
Section
License
Copyright (c) 2025 Ubaidillah Bramantyo Esnandhik, Fatimah Azzahra, Ni Ketut Sari
This work is licensed under a Creative Commons Attribution 4.0 International License.
