Potential and Characterization of Yeast from Soil Contaminated with Captan – Based Fungicide
DOI:
https://doi.org/10.70609/g-tech.v9i3.7253Keywords:
Bioremediation, Yeast, Fungicide Residues, CaptanAbstract
Captan is a fungicide that inhibits the growth of fungi such as Alternaria porri. This fungicide is non-systemic, forming a layer on the surface of leaves and fruit. The continuous use of captan fungicides can leave residues in the environment, especially in soil. One technique to reduce captan fungicide residues is bioremediation. Bioremediation is a way to remove fungicide residues by utilizing microorganisms. The purpose of the study was to obtain yeasts in land polluted with residues of fungicides made from active captan, knowing yeasts that are adaptive to various fungicide concentrations and have the potential to reduce the toxicity of fungicides made from active captan. This study includes soil sampling, isolation and characterization of yeasts morphologically and molecularly, yeast adaptation tests to fungicides, and degradation tests using a complete randomized design. In the adaptation test, all yeasts were able to grow on media added with various concentrations of fungicide product recommendations. Molecular identification of yeast includes DNA isolation, DNA amplification, PCR product sequencing, and molecular data analysis. In the treatment with the addition of yeast, the largest average diameter of A. porri on the seventh day of observation was in the treatment with the addition of W. anomalus and W. onychis yeast. The yeast with the highest ability to degrade fungicides containing the active ingredient captan is W. onychis.
References
Aibeche, C., Selami, N., Zitouni-Haouar, F. E.-H., Oeunzar, K., Addou, A., Kaid-Harche, M., & Djabeur, A. (2022). Bioremediation potential and lead removal capacity of heavy metal-tolerant yeasts isolated from Dayet Oum Ghellaz Lake water (northwest of Algeria). International Microbiology, 25(1), 61–73. https://doi.org/10.1007/s10123-021-00191-z
Al Halim, L. R. A., Hemeda, N. F., & Serag, A. M. (2024). Isolation, characterization, and screening of yeast biodiversity for multi- hydrolytic enzymes. Journal of Umm Al-Qura University for Applied Sciences, 10(3), 474–484. https://doi.org/10.1007/s43994-023-00118-6
Alori, E. T., Gabasawa, A. I., Elenwo, C. E., & Agbeyegbe, O. O. (2022). Bioremediation techniques as affected by limiting factors in soil environment. Frontiers in Soil Science, 2. https://doi.org/10.3389/fsoil.2022.937186
Ataikiru, T. L., Okorhi-Damisa, B. F., & Akpaiboh, J. I. (2017). Microbial community structure of an oil polluted site in Effurun, Nigeria. International Research Journal of Public and Environmental Health, 4(3), 41–47. https://doi.org/10.15739/irjpeh.17.006
Bessadok, B., Jaouadi, B., Brück, T., Santulli, A., Messina, C. M., & Sadok, S. (2022). Molecular Identification and Biochemical Characterization of Novel Marine Yeast Strains with Potential Application in Industrial Biotechnology. Fermentation, 8(10), 538. https://doi.org/10.3390/fermentation8100538
Cai, M., Zhou, J., Hao, T., & Du, K. (2022). Tolerance of phyllospheric Wickerhamomyces anomalus to BDE-3 and heavy metals. Environmental Science and Pollution Research, 29(37), 56555–56561. https://doi.org/10.1007/s11356-022-19798-4
Dubois, T., Hadi, B. A. R., Vermeulen, S., Ballantyne, P., Dobermann, A., Fan, S., Garrett, K. A., Ibabao, X., Ismail, A., Jaramillo, J., Loboguerrero, A. M., McCutcheon, S., Njuki, J., Sharma, T. R., Tonnang, H. E. Z., & Pede, V. (2024). Climate change and plant health: impact, implications and the role of research for mitigation and adaptation. Global Food Security, 41, 100750. https://doi.org/10.1016/J.GFS.2024.100750
Ebadi, T., Najafpour, G. D., Younesi, H., & Mohammadi, M. (2022). Rapid biodegradation of diazinon using a novel strain of Candida pseudolambica. Environmental Technology and Innovation, 25. https://doi.org/10.1016/j.eti.2021.102218
Elsamahy, T., Sun, J., Elsilk, S. E., & Ali, S. S. (2023). Biodegradation of low-density polyethylene plastic waste by a constructed tri-culture yeast consortium from wood-feeding termite: Degradation mechanism and pathway. Journal of Hazardous Materials, 448. https://doi.org/10.1016/j.jhazmat.2023.130944
Gama, A. B., Cordova, L. G., Baggio, J. S., Mertely, J. C., & Peres, N. A. (2023). Old but Gold: Captan Is a Valuable Tool for Managing Anthracnose and Botrytis Fruit Rots and Improving Strawberry Yields Based on a Meta-Analysis. Plant Disease, 107(10), 3071–3078. https://doi.org/10.1094/PDIS-12-22-2781-RE
García-Béjar, B., Arévalo-Villena, M., Guisantes-Batan, E., Rodríguez-Flores, J., & Briones, A. (2020). Study of the bioremediatory capacity of wild yeasts. Scientific Reports, 10(1), 11265. https://doi.org/10.1038/s41598-020-68154-4
Hashem, M., Alamri, S. A., Al-Zomyh, S. S. A. A., & Alrumman, S. A. (2018). Biodegradation and detoxification of aliphatic and aromatic hydrocarbons by new yeast strains. Ecotoxicology and Environmental Safety, 151, 28–34. https://doi.org/10.1016/j.ecoenv.2017.12.064
Ibrahim A. Mirsal. (2008). Soil Pollution. Springer Berlin Heidelberg. https://doi.org/10.1007/978-3-540-70777-6
Kim, K. H., Kabir, E., & Jahan, S. A. (2017). Exposure to pesticides and the associated human health effects. Science of The Total Environment, 575, 525–535. https://doi.org/10.1016/J.SCITOTENV.2016.09.009
Megadi, V. B., Tallur, P. N., Mulla, S. I., & Ninnekar, H. Z. (2010). Bacterial degradation of fungicide captan. Journal of Agricultural and Food Chemistry, 58(24), 12863–12868. https://doi.org/10.1021/jf1030339
Mohiuddin, O., P. Harvey, A., Orta Ledesma, M. T., & Velasquez-Orta, S. (2024). Bioremediation of waste by yeast strains. Electronic Journal of Biotechnology, 69, 30–42. https://doi.org/10.1016/j.ejbt.2024.01.005
Rana, S., Handa, S., Aggarwal, Y., Puri, S., & Chatterjee, M. (2023). Role of Candida in the bioremediation of pollutants: a review. Letters in Applied Microbiology, 76(9). https://doi.org/10.1093/lambio/ovad103
Simanjuntak, D., Faizah, R., Prasetyo, A. E., & Susanto, A. (2017). Keefektifan Fungisida Terhadap Isolat Cendawan Terbawa Benih Kelapa Sawit The Effectiveness Of Fungicides Against Fungal Isolates Carried By Oil Palm Seeds.
Subair Gaffar Baso, M., Hasanah, U., & Monde, A. (2014). Variabilitas Sifat Fisika Tanah dan C-Organik Pada Lahan Hutan dan Perkebunan Kakao (Theobroma cacao L.) di Desa Sejahtera Kecamatan Palolo Kabupaten Sigi.
Wang, X.-X., Zhao, Z.-H., Chang, T.-S., & Liu, J.-G. (2011). Yeast screening from avermectins wastewater and investigation on the ability of its fermentation. Bioprocess and Biosystems Engineering, 34(9), 1127–1132. https://doi.org/10.1007/s00449-011-0563-6
Yurkov, A. M. (2018). Yeasts of the soil – obscure but precious. In Yeast (Vol. 35, Issue 5). John Wiley and Sons Ltd. https://doi.org/10.1002/yea.3310
Zhang, L., Zuo, Q., Cai, H., Li, S., Shen, Z., & Song, T. (2024). Fungicides reduce soil microbial diversity, network stability and complexity in wheat fields with different disease resistance. Applied Soil Ecology, 201, 105513. https://doi.org/10.1016/J.APSOIL.2024.105513
Downloads
Published
Issue
Section
License
Copyright (c) 2025 Ratna Eka Sari Putri, Chosa Zahro Fatiha, Novia Dwi Putri
This work is licensed under a Creative Commons Attribution 4.0 International License.
